Part:BBa_K3396005:Design

Trim21-DocS

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 204
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 161
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We repleced the antigen-antibody interaction with DocS-Coh2 interaction to keep the binding of TRIM21 and the GFPnano. And we truncated the TRIM21 and replaced its PRYSPRY domain with DocS.

Source

Trim21 is originally expressed in human and DocS is originally expressed in C. thermocellum scaffoldin